Blood sampling sequence recommendations
Blood sampling sequence* | Cap color** | Tube type |
Number of shuffles |
Minimum clotting time |
Centrifugation Conditions |
The maximum time before the study |
Without additives | Empty tubes | 5–6 | 60 min. | 1800 g 10 minutes. in 25°С | 3 h | |
Study of the hemostatic system | Trisodium citrate tubes (1: 9) | 3–4 | – | 2000–2500 g 10-15 minutes. in 25°C | 3 h | |
ESR determination | ESR tubes with trisodium citrate (1: 4) | 8–10 | – | – | 3 h | |
Clinical chemistry, serology, ELISA, bacteriology, for determination of blood group | Test tubes for serum with silica or thrombin | 5–6 | 30 minutes. for silica 5 min. for thrombin | 1800 g 10 minutes. при 25°С | 3 h | |
Test tubes for serum with silica or thrombin with separation gel | 5–6 | 30 minutes. for silica 5 min. for thrombin |
2500–3000 g 10–15 minutes. in 25°С
|
2 hours before centrifugation and 48 hours after centrifugation | ||
Biochemistry, immunology (vitamins, hormones, immune status) | Lithium heparin or sodium heparin | 8–10 | – | 1800 g 10 minutes. in 25°С | 3 h | |
Lithium heparin or sodium heparin with separation gel | 2500-3000 g 10–15 minutes. in 25°С | 2 hours before centrifugation and 48 hours after centrifugation | ||||
Hematology, immunochemistry, PCR, genodiagnosis | test tube with EDTA | 8–10 | – | – |
24 h
|
|
Molecular identification of viruses, parasitic microorganisms and bacteria | test tube with EDTA and separation gel | 2500-3000 g 10-15 minutes. in 25°С | 6 h | |||
Determination of glucose and lactate levels in diabetology | Test tubes with sodium fluoride and potassium oxalate | 8–10 | – | 1800 g 10 minutes. in. 25°С | 48 h | |
Test tubes with sodium fluoride and EDTA |