Blood sampling sequence recommendations
Blood sampling sequence* | Cap color** | Tube type |
Number of shuffles |
Minimum clotting time |
Centrifugation Conditions |
The maximum time before the study |
Without additives |
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Empty tubes | 5–6 | 60 min. | 1800 g 10 minutes. in 25°С | 3 h |
Study of the hemostatic system |
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Trisodium citrate tubes (1: 9) | 3–4 | – | 2000–2500 g 10-15 minutes. in 25°C | 3 h |
ESR determination |
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ESR tubes with trisodium citrate (1: 4) | 8–10 | – | – | 3 h |
Clinical chemistry, serology, ELISA, bacteriology, for determination of blood group |
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Test tubes for serum with silica or thrombin | 5–6 | 30 minutes. for silica 5 min. for thrombin | 1800 g 10 minutes. при 25°С | 3 h |
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Test tubes for serum with silica or thrombin with separation gel | 5–6 | 30 minutes. for silica 5 min. for thrombin |
2500–3000 g 10–15 minutes. in 25°С
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2 hours before centrifugation and 48 hours after centrifugation | |
Biochemistry, immunology (vitamins, hormones, immune status) |
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Lithium heparin or sodium heparin | 8–10 | – | 1800 g 10 minutes. in 25°С | 3 h |
Lithium heparin or sodium heparin with separation gel | 2500-3000 g 10–15 minutes. in 25°С | 2 hours before centrifugation and 48 hours after centrifugation | ||||
Hematology, immunochemistry, PCR, genodiagnosis |
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test tube with EDTA | 8–10 | – | – |
24 h
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Molecular identification of viruses, parasitic microorganisms and bacteria | test tube with EDTA and separation gel | 2500-3000 g 10-15 minutes. in 25°С | 6 h | |||
Determination of glucose and lactate levels in diabetology |
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Test tubes with sodium fluoride and potassium oxalate | 8–10 | – | 1800 g 10 minutes. in. 25°С | 48 h |
Test tubes with sodium fluoride and EDTA |